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Series GSE8368

Query DataSets for GSE8368

Status

Public on Aug 07, 2008

Title

Isolation and transcriptional profiling of embryonic human neural crest cells

Organism

Homo sapiens

Experiment type

Expression profiling by SAGE

Summary

To identify new candidate genes for neurocristopathies, a Long-SAGE library has been made from human neural crest cells (hNCC) derived from a normal embryo at Carnegie stage 13. Data analysis indicates the expression of a number of genes in multiple GO functional classes that were anticipated based on animal studies and/or human disease. An average linkage hierarchical clustering was performed using SAGE data from hNCC and 14 other normal tissues and cell lines. This and other analyses indicate a high degree of molecular identity between hNCC and the two human embryonic stem cells (hES) lines included, compared to the other tissue or cell types included, including more tissue-restricted stem cells, such as mesenchymal stem cells, or hNCC derivatives such as Schwann cells.
Keywords: Cell type comparison

Overall design

Total RNA was extracted from a confluent 10 cm dish of hNCC (line N5) from a female human embryo at 28-32 dpf (Carnegie stage 13). Half the RNA was used to construct a SAGE bank with long tags using NlaIII and MmeI. Data was then compared to currently publicly available SAGE data from fourteen banks made from normal human tissues for hierarchical clustering.
Third-party GEO accession numbers for human SAGE data:
GSM41360, hES3;
GSM41362, hES4;
GSM48250, sciatic nerve;
GSM48251, Schwann cells (in vitro);
GSM31931, brain substantia nigra;
GSM824, muscle;
GSM785, liver;
GSM708, kidney;
GSM676, brain white matter;
GSM761, brain cerebellum;
GSM762, lung.

Other third-party data:
Cancer Genome Anatomy Project http://cgap.nci.nih.gov/SAGE/SAGELibraryFinder: SAGE_Prostate_normal_B_2 (prostate)

Umbilical cord- and bone marrow-derived mesenchymal stem cell data had been downloaded from currently broken links http://bit.fmrp.usp.br/uc-msc_tags/ and http://bit.fmrp.usp.br/msc_tags/ as referenced in Panepucci et al. (2004) Stem Cells 22:1263-1278; DOI: 10.1634/stemcells.2004-0024 (UC-MSC) and Silva et al. (2003) Stem Cells 21:661-669.

Contributor(s)

Thomas S, Thomas M, Wincker P, Xu P, Speer MC, Munnich A, Lyonnet S, Vekemans M, Etchevers HC, Babarit C

Citation(s)

18689800

Submission date

Jul 03, 2007

Last update date

Sep 21, 2012

Contact name

Heather C Etchevers

E-mail(s)

heather.etchevers@inserm.fr