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| Status |
Public on Sep 20, 2016 |
| Title |
ADDITIVE EFFECTS OF MILLIMETER WAVES AND 2 DEOXYGLUCOSE CO-EXPOSURE ON THE HUMAN KERATINOCYTE TRANSCRIPTOME |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To evaluate the effect of millimeter waves (MMW) exposure on the gene expression, we have employed whole genome microarray expression profiling. Neonatal primary Keratinocyte cells pooled from 3 healthy donors were exposed ex vivo, by 60.4 GHz-MMW, at an incident power density (IPD) of 20 mW/cm² (3 hours of treatment in athermic condition).No modification of keratinocyte transcriptome was observed. Considering that MMW could also impact the cell metabolism, we assessed then effect of glycolysis inhibitor 2 deoxyglucose treatment (2dG, 20 mM during 3 hours) in association with MMW co-exposure. 2dG treatment induces a high modification of the transcriptome (632 coding genes). Genes affected in their expression are linked with transcriptional repression, cellular communication and endoplasmic reticulum homeostasis. The MMW/2dG co-exposition induced a slight modification of cell transcriptome, which reflects the capacity of MMW to interfere with bioenergetic stress response. After RT-PCR validation, 6 genes (SOCS3, SPRY2, TRIB1, FAM46A, CSRNP1 and PPP1R15A) were confirmed as sensitive to MMW exposure during 2dG treatment.
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| Overall design |
Gene expression in human keratinocytes was measured after a 60 GHz-exposure at 20 mW/cm2, for 3 h (Expo). It was compared to cells cultured in unexposed condition (Sham). As millimeter wave (MMW) exposure impact on metabolism we assessed then effect of glycolysis inhibitor 2 deoxyglucose treatment (2dG, 20 mM during 3 hours: Sham_2dG) in association with MMW co-exposure (Expo_2dG).
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| Contributor(s) |
Mahamoud YS, Aite M, Martin C, Zhadobov M, Sauleau R, Le Dréan Y, Habauzit D |
| Citation(s) |
27529420 |
| Submission date |
Jun 28, 2016 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Denis Habauzit |
| Organization name |
ANSES
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| Department |
TC unit
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| Street address |
10 B rue Claude Bourgelat
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| City |
Fougeres |
| ZIP/Postal code |
35306 |
| Country |
France |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA327119 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE83829_RAW.tar |
49.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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