 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 01, 2016 |
| Title |
Characterization of active piRNAs and an piRNA machinery in WRR-1 cells deficient for the Hippo pathway (deficient for the warts tumor suppressor) and expressing activated RasV12 (small RNA) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We have found that oncogenic Ras combined with loss of the Hippo tumour-suppressor pathway reactivates a primary piRNA pathway in Drosophila somatic cells, coincident with oncogenic transformation. In these cells, Piwi becomes loaded with piRNAs derived from generative loci, which are normally restricted to either the germline or the somatic follicle cells. Negating the pathway leads to increases in the expression of a wide variety of transposons and also to altered expression of some protein coding genes. This correlates with a reduction in the proliferation of the transformed cells in culture, suggesting that at least in this context, the piRNA pathway may play a functional role in cancer.
|
| |
|
| Overall design |
Small RNAs cloned from whole cells, piwi-bound small RNAs and long RNA-Seq were performed in wts-RNAi;RasV12 cells to identify piRNAs and gene expression and compared to RasV12 cells, somatic OSS cells, and germline UAS-wts-RNAi;UAS-RasV12 control ovaries. After knock-down of several components of the piRNA machinery in WRR-1 cells (piwi, zuchini, armitage, aubergine, argonaute3) and GFP and/or dsRED control knock-downs, small RNAs were cloned and gene expression profiles (RNA-Seq) were established.
|
| |
|
| Contributor(s) |
Fagegaltier D, Hannon GJ |
| Citation(s) |
27474441 |
| Submission date |
Jun 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Delphine Fagegaltier |
| Organization name |
Cold Spring Harbor Lab
|
| Department |
Genomics Division
|
| Lab |
Greg Hannon
|
| Street address |
One Bungtown Rd
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
|
| Samples (18)
|
| GSM2220189 |
total small RNAs (19-28nt) from UAS-wts-RNAi, UAS-RasV12 adult ovaries |
| GSM2220190 |
total small RNAs (19-28nt) from OSS cells |
| GSM2220191 |
total small RNAs (19-28nt) from WRR-1 cells |
| GSM2220192 |
piwi-bound small RNAs (19-28nt) from OSS cells, replicate 1 |
| GSM2220193 |
piwi-bound small RNAs (19-28nt) from OSS cells, replicate 2 |
| GSM2220194 |
piwi-bound small RNAs (19-28nt) from WRR-1 cells, replicate 1 |
| GSM2220195 |
piwi-bound small RNAs (19-28nt) from WRR-1 cells, replicate 2 |
| GSM2220196 |
total small RNAs (19-28nt) from WRR-1 GFP control knock-down cells, replicate 1 |
| GSM2220197 |
total small RNAs (19-28nt) from WRR-1 GFP control knock-down cells, replicate 2 |
| GSM2220198 |
total small RNAs (19-28nt) from WRR-1 piwi knock-down cells, replicate 1 |
| GSM2220199 |
total small RNAs (19-28nt) from WRR-1 piwi knock-down cells, replicate 2 |
| GSM2220200 |
total small RNAs (19-28nt) from WRR-1 zucchini knock-down cells, replicate 1 |
| GSM2220201 |
total small RNAs (19-28nt) from WRR-1 zucchini knock-down cells, replicate 2 |
| GSM2220202 |
total small RNAs (19-28nt) from WRR-1 armitagei knock-down cells, replicate 1 |
| GSM2220203 |
total small RNAs (19-28nt) from WRR-1 armitagei knock-down cells, replicate 2 |
| GSM2220204 |
total small RNAs (19-28nt) from WRR-1 aubergine knock-down cells, replicate 1 |
| GSM2220205 |
total small RNAs (19-28nt) from WRR-1 aubergine knock-down cells, replicate 2 |
| GSM2220206 |
total small RNAs (19-28nt) from WRR-1 argonaute3 knock-down cells, replicate 1 |
|
| This SubSeries is part of SuperSeries: |
| GSE83877 |
Characterization of active piRNAs and piRNA machinery in WRR-1 cells deficient for the Hippo pathway (deficient for the warts tumor suppressor) and expressing activated RasV12 |
|
| Relations |
| BioProject |
PRJNA327243 |
| SRA |
SRP077585 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE83871_RAW.tar |
20.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...