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| Status |
Public on Aug 15, 2016 |
| Title |
Exosomal microRNA, miR-1246, induces cell motility and invasion through the regulation of DENND2D in oral squamous cell carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
To Identify of metastasis-related genes and metastasis-related miRNA in oral squamous cell carcinoma by miRNA profiling of HOC313-Parent, HOC313-LM and their respective exosomes carried out. As a result, miR-342-3p and -1246 were found candidate oncogenic miRNAs.
To further identify the target genes of miR-1246 and miR-342-3p, we performed gene expression array analysis with HOC313-LM and HOC313-Parent (HOC313-P) transfected NT, miR-342-3p and miR-1246. Moreover, to identify tumor suppressor genes, gene expression profiles of each of transfected cells and HOC313-LM cells were analyzed by in silico analyses. As a result, DENND2D emerged as the possible target of miRN-1246.
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| Overall design |
Extraction of difference of miRNA expression level between whole cell and exosomes of HOC313-Parent and HOC313-LM. Also, extraction of difference of gene expression level between high-metastatic subline (HOC313-LM) and low-metastatic subline (HOC313-P). Moreover the miRNA expression profiles and gene expression profiles were analyzed by in silico analyses.
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| Contributor(s) |
Sakha S, Muramatsu T, Inazawa J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 04, 2016 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Tomoki Muramatsu |
| E-mail(s) |
muramatsutomoki@gmail.com
|
| Phone |
+81-3-5803-5821
|
| Organization name |
Tokyo Medical and Dental University
|
| Department |
Medical research institute
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| Lab |
Molecular cytogenesis
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| Street address |
1-5-45 Yushima Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-8510 |
| Country |
Japan |
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| Platforms (2) |
| GPL11487 |
Agilent-021827 Human miRNA Microarray [miRNA_107_Sep09_2_105] |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
|
| Samples (16)
|
| GSM2224822 |
HOC313-P whole cell miRNA expression 1 |
| GSM2224823 |
HOC313-P whole cell miRNA expression 2 |
| GSM2224824 |
HOC313-LM whole cell miRNA expression 1 |
| GSM2224825 |
HOC313-LM whole cell miRNA expression 2 |
| GSM2224826 |
HOC313-P exosomes miRNA expression 1 |
| GSM2224827 |
HOC313-P exosomes miRNA expression 2 |
| GSM2224828 |
HOC313-LM exosomes miRNA expression 1 |
| GSM2224829 |
HOC313-LM exosomes miRNA expression 2 |
| GSM2224830 |
HOC313-P Negative control gene expression 1 |
| GSM2224831 |
HOC313-P Negative control gene expression 2 |
| GSM2224832 |
HOC313-P overexpressed with miR-342-3p gene expression 1 |
| GSM2224833 |
HOC313-P overexpressed with miR-342-3p gene expression 2 |
| GSM2224834 |
HOC313-P overexpressed with miR-1246 gene expression 1 |
| GSM2224835 |
HOC313-P overexpressed with miR-1246 gene expression 2 |
| GSM2224836 |
HOC313-LM whole cell gene expression 1 |
| GSM2224837 |
HOC313-LM whole cell gene expression 2 |
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| Relations |
| BioProject |
PRJNA327741 |
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