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| Status |
Public on Jul 31, 2018 |
| Title |
Hepatic miRNA profile in dengue hemorrhagic fever associated with apoptosis regulation, vascular injury and inflammation |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Purpose: Dengue virus (DENV) causes dengue hemorrhagic fever (DHF) and have been often associated to fatal cases worldwide. The liver is one of the most important target tissues in severe cases, due to its intense viral replication and role in metabolism. microRNAs (miRNA) role during infection is crucial to understand the regulatory mechanisms of DENV infection, and can help in diagnostic and anti-viral therapies development.
Methods: miRNoma sequencing of ten fatal cases and compared to controls, to characterize the miRNAs expression profile of the human formalin fixed paraffin embedded (FFPE) liver tissue during DHF
Results: Eight miRNAs were differentially expressed in hepatic FFPE tissue: miR-126-5p (log2FC 3.09; p< 0,001), a regulatory molecule of endothelial cells, and miR-133a-3p (100-fold, p<0,001) were up regulated in DHF and miR-122-5p (a liver-specific miRNA), miR-146a-5p (Interferon-regulator), miR-10b-5p, miR-204-5p, miR-148a-5p and miR-423-5p were down regulated. Enrichment analysis of KEGG pathways and GO terms with predicted target genes of overexpressed miRNAs revealed regulatory pathways of apoptosis, involving MAPK, RAS, CDK and FAS and immune response pathways were related to NF- kB, CC and CX families, IL and TLR. The enrichment analysis using target genes of down regulated miRNAs in DHF also identified in most pathways of apoptosis and biosynthetic pathways.
Conclusions: This is the first description of the human miRNA profile in liver tissues from DHF cases. The results demonstrated the association of miR-126-5p, miR-122-5p and miR-146a-5p with DHF liver pathogenesis, involving endothelial repair and vascular permeability regulation, control of homeostasis and expression of inflammatory cytokines.
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| Overall design |
miRNA profile from ten FFPE hepatic tissue of dengue hemorragic fever fatal cases compared with five control FFPE tissue from non hepatic injury individuals sequenced by MiSeq Illumina.
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| Contributor(s) |
Oliveira LF, Vianez JL, Pagliari C, Carvalho LV, Silveira TS, Cardoso JF, Telles AL, Vasconcelos JM, Moreira-Nunes CA, Burbano RR, Nunes MT, Santos EM |
| Citation missing |
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| Submission date |
Jul 18, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Layanna Freitas de Oliveira |
| E-mail(s) |
layannafoliveira@gmail.com
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| Organization name |
Evandro Chagas Institute
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| Department |
Arbovirology Section
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| Lab |
Center of Innovation and Technology
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| Street address |
BR 316 Km 7
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| City |
Ananindeua |
| State/province |
Pará |
| ZIP/Postal code |
67030-000 |
| Country |
Brazil |
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| Platforms (1) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA329550 |
| SRA |
SRP078861 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE84536_DE-ResultsDhfVsControls.csv.gz |
97.4 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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