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| Status |
Public on Feb 15, 2017 |
| Title |
Gene expression analysis of human macrophages exposed to lipoprotein lipase hydrolysis products |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Lipoprotein lipase (LPL) is an extracellular lipase that preferentially hydrolyses triglycerides in triglyceride-rich lipoproteins within the circulation. LPL expression in macrophages contributes to atherosclerosis. In addition, the hydrolysis products liberated from lipoprotein lipids by LPL causes lipid accumulation and impairs cholesterol efflux ability in macrophages. However, the effects of LPL hydrolysis products in modulating the transcript profiles within macrophages and their roles in foam cell formation are not completely understood.
We performed microarray analyses on THP-1 macrophages incubated with LPL hydrolysis products to identify differentially expressed genes.
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| Overall design |
Human LPL was recombinantly expressed in HEK-293T cells. Heparinized media from cells expressing LPL or from control cells were used to hydrolyse total lipoproteins that were isolated from normolipidemic subjects. THP-1 macrophages were incubated with 0.68 mM (by unesterified fatty acid content) of LPL hydrolysis products, or non-hydrolysis products (of 0.03 mM by unesterified fatty acid content) for 18 hours (n=3). Total RNA was isolated from treated samples and it was used for human gene arrays.
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| Contributor(s) |
Thyagarajan N, Christian SL, Brown RJ |
| Citation(s) |
28205069 |
| Submission date |
Jul 25, 2016 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Sherri L Christian |
| E-mail(s) |
sherri@mun.ca
|
| Phone |
(709)864-8550
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| Organization name |
Memorial University of Newfoundland
|
| Department |
Biochemistry
|
| Street address |
232 Elizabeth Ave
|
| City |
St. John's |
| State/province |
Newfoundland |
| ZIP/Postal code |
A1B3X9 |
| Country |
Canada |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM2251412 |
THP-1 macrophages incubated with non-hydrolysis products_replicate 1 |
| GSM2251413 |
THP-1 macrophages incubated with non-hydrolysis products_replicate 2 |
| GSM2251414 |
THP-1 macrophages incubated with non-hydrolysis products_replicate 3 |
| GSM2251415 |
THP-1 macrophages incubated with LPL hydrolysis products_replicate 1 |
| GSM2251416 |
THP-1 macrophages incubated with LPL hydrolysis products_replicate 2 |
| GSM2251417 |
THP-1 macrophages incubated with LPL hydrolysis products_replicate 3 |
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| Relations |
| BioProject |
PRJNA331149 |
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