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| Status |
Public on Feb 20, 2017 |
| Title |
CAN1 Arginine Permease Deficiency Extends Yeast Replicative Lifespan via Translational Activation of Stress Response Genes |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptional regulation plays an important role in the control of gene expression during aging. However, translation efficiency likely plays an equally important role in determining protein abundance, but has been relatively under studied in this context. Here we used RNA-seq and ribosome profiling to investigate the role of translational regulation in lifespan extension by CAN1 gene deletion in yeast. Through comparison of the transcriptional and translational changes in cells lacking CAN1 with other long-lived mutants, we were able to identify critical regulatory factors, including transcription factors and mRNA-binding proteins, that coordinate transcriptional and translational responses. Together, our data support a model in which deletion of CAN1 extends replicative lifespan through increased translation of proteins that facilitate cellular response to stress. This study extends our understanding of the importance of translational control in regulating stress resistance and longevity.
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| Overall design |
RNA-seq and ribosome profiling analyses of wild-type, can1, rpl22a, and tor1 knockout strains
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| Contributor(s) |
Beaupere C, Wasko BM, Lorusso J, Kennedy BK, Kaeberlein M, Labunskyy VM |
| Citation(s) |
28228255 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R00 AG040191 |
Endoplasmic Reticulum Thiol Redox State and Unfolded Protein Response in Aging |
BOSTON UNIVERSITY |
Vyacheslav M Labunskyy |
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| Submission date |
Aug 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Vyacheslav Labunskyy |
| E-mail(s) |
vlabuns@bu.edu
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| Organization name |
Boston University School of Medicine
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| Department |
Department of Dermatology
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| Street address |
609 Albany Street
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (16)
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| GSM2260109 |
can1Δ ribo-seq, replicate 1 |
| GSM2260110 |
can1Δ ribo-seq, replicate 2 |
| GSM2260111 |
can1Δ ribo-seq, replicate 3 |
| GSM2260112 |
wild-type RNA-seq, replicate 1 |
| GSM2260113 |
wild-type RNA-seq, replicate 2 |
| GSM2260114 |
can1Δ RNA-seq, replicate 1 |
| GSM2260115 |
can1Δ RNA-seq, replicate 2 |
| GSM2260116 |
rpl22aΔ ribo-seq, replicate 1 |
| GSM2260117 |
rpl22aΔ ribo-seq, replicate 2 |
| GSM2260118 |
tor1Δ ribo-seq, replicate 1 |
| GSM2260119 |
tor1Δ ribo-seq, replicate 2 |
| GSM2260120 |
rpl22aΔ RNA-seq |
| GSM2260121 |
tor1Δ RNA-seq |
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| Relations |
| BioProject |
PRJNA336542 |
| SRA |
SRP080941 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85198_RAW.tar |
990.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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