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| Status |
Public on Dec 01, 2020 |
| Title |
PHF6 keeps hematopoietic lineage development in check [Jurkat] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of the mutational landscape of various hematological malignancies has shown that key regulators implicated in lineage commitment and differentiation are frequently implicated in leukemic transformation. In T-cell acute lymphoblastic leukemia, the role of various oncogenic and tumor suppressing transcription factors has been investigated. More recently, an exceptional high prevalence of mutations affecting epigenetic modifiers in T-ALL has also been noted. In order to understand the exact contribution of such lesions to normal differentiation and oncogenic transformation, further functional characterization of this subclass of proteins is warranted. PHF6 is one of these key epigenetic players, which was described as a frequently mutated and deleted novel tumor suppressor in T-ALL. In a first step towards unraveling the role of PHF6 in normal and malignant hematopoiesis, we characterized the function of PHF6 in both normal T-cell differentiation and other hematopoietic lineages as a prelude to understand its contribution to leukemogenesis. Here, we show for the first time that PHF6 controlled epigenetic regulation positively controls a NOTCH1 gene signature and the effects observed on T-lineage differentiation seem to be additive with Notch1. In this study, we show for the first time that PHF6 is a master regulator of early hematopoietic differentiation along the T-cell, B-cell, myeloid and NK-cell lineages.
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| Overall design |
Gene expression was measured in Jurkat T-ALL lymphoblasts upon lentiviral infetion with scrambled or a PHF6 targeting shRNA. Cells were collected after 96h post-transduction. This was performed for 3 independent biological replicates.
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| Contributor(s) |
Durinck K, Loontiens S, Van de Walle I, Van Vlierberghe P, Speleman F, Taghon T |
| Citation(s) |
33251223 |
| Submission date |
Aug 09, 2016 |
| Last update date |
Dec 01, 2020 |
| Contact name |
Kaat Durinck |
| E-mail(s) |
kaat.durinck@ugent.be
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| Phone |
003293322453
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| Organization name |
Center for Medical Genetics
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| Department |
Biomolecular Medicine
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| Street address |
Corneel Heymanslaan 10
|
| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE85373 |
PHF6 is a key regulator of normal human hematopoiesis by modulating NOTCH1 signaling activity |
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| Relations |
| BioProject |
PRJNA338292 |
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