|
| Status |
Public on Aug 17, 2016 |
| Title |
The effect of IKKβ on estrogen receptor positive breast cancer phenotypes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Estrogen receptor α (ER) is a good prognostic marker expressed in ~75% of breast tumors. Women with ER+ tumors will receive endocrine therapy, yet ~50% will experience relapse and late recurrence ~5-20 years after primary diagnosis.
The recurrent tumors are often aggressive, resistant, and metastatic. A growing body of evidence suggests that an inflammatory microenvironment and the activation of the NF-ĸB pathway are highly associated with the progression of ER+ tumors
to more aggressive stages. However, it is unknown whether NF-ĸB is a driver or a consequence of aggressive ER+ disease. In order to study this, we developed multiple ER+ breast cancer cell lines expressing a Doxycycline-inducible, constitutively
active form of IĸB kinase β (CA-IKKβ), a key kinase in the canonical NF-ĸB pathway. This allowed us to specifically activate the canonical arm of the NF-ĸB pathway in a controlled fashion.
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| |
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| Overall design |
Constitutively active IKK β-MCF-7 cells are treated with Estrogen (E2), Doxycycline (Dox) or E2+Dox for 72 hrs.The study includes four groups: Vehicle, E2, Dox and E2+Dox with three replicates per group.
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| |
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| Contributor(s) |
Frasor J, El-Shennawy L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 16, 2016 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Jonna Frasor |
| E-mail(s) |
jfrasor@uic.edu
|
| Organization name |
University of Illinois at Chicago
|
| Department |
Physiology and Biophysics
|
| Lab |
Jonna Frasor
|
| Street address |
835 S. Wolcott MC901
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
|
| Samples (12)
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|
| Relations |
| BioProject |
PRJNA339054 |
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