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| Status |
Public on Dec 22, 2016 |
| Title |
Expression data from BT-474 cells long-term treated with everolimus |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Inhibitors of the mechanistic target of rapamycin (mTOR) are currently used to treat advanced metastatic breast cancer. However, whether an aggressive phenotype is sustained through adaptation or resistance to mTOR inhibition remains unknown. Here, complementary studies in human tumors, cancer models and cell lines reveal transcriptional reprogramming that supports metastasis in response to mTOR inhibition. This cancer feature is driven by EVI1 and SOX9. EVI1 functionally cooperates with and positively regulates SOX9, and promotes the transcriptional upregulation of key mTOR pathway components (REHB and RAPTOR) and of lung metastasis mediators (FSCN1 and SPARC). The expression of EVI1 and SOX9 is associated with stem cell-like and metastasis signatures, and their depletion impairs the metastatic potential of breast cancer cells. These results establish the mechanistic link between resistance to mTOR inhibition and cancer metastatic potential, thus enhancing our understanding of mTOR targeting failure.
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| Overall design |
BT-474 cell cultures were treated with 150 nM everolimus for approximately 100 days and subsequently RNA extracted, processed and hybridized on Affymetrix microarrays
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| Contributor(s) |
Pujana MA, Mateo F |
| Citation(s) |
27991928 |
| Submission date |
Aug 18, 2016 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Miguel Angel Pujana |
| E-mail(s) |
mapujana@iconcologia.net
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| Phone |
0034932607463
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| Organization name |
Institut Catala d'Oncologia IDIBELL
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| Lab |
ProCURE
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| Street address |
Gran Via 199
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| City |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA339428 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85801_RAW.tar |
4.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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