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| Status |
Public on Jul 01, 2018 |
| Title |
BORIS knockdown in colorectal cancer cells HCT116 and Caco-2 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
BORIS expresses abnormally in colorectal cancer cells. Both the expression and the copy number of BORIS are remarkably higher in colorectal cancer cells than in normal colon or rectal cells. BORIS is potential diagnosis, prognosis or therapeutic target for colorectal cancer. To expand our view of the signaling pathway related with BORIS, altered gene expression by BORIS knockdown was assessed by microarray assay.
To study the gene regulation by BORIS knockdown, microarray assay was applied to screen the gene expression regulated by BORIS siRNA in colorectal cancer cells HCT116 and Caco-2
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| Overall design |
HCT116 and Caco-2 cells were silenced by siRNA of BORIS for 3 days and extracted for the RNA. Silenced samples and the controls were compared to screen for gene expression difference by Affymetrix PrimeView human gene expression array.
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| Contributor(s) |
Zhang Y, Fang M, Ren J, Song Y, Yuan H, Fang J, Yan D, Wang X |
| Citation(s) |
31949703 |
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Title: Atractyloside mimics BORIS knockdown to induce DNA damage incolorectal cancer cells
Journal: International Journal ofClinical and Experimental Pathology; Issue: 2018;11(7), page numbers: 3286-3293
Authors: Mengdie Fang, Yongfei Song, Juan Ren, Haining Yuan, JianfeiFang, Dongmei Yan, Yanmei Zhang, Xiaoju Wang
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| Submission date |
Aug 29, 2016 |
| Last update date |
Jan 21, 2020 |
| Contact name |
Yanmei Zhang |
| E-mail(s) |
yanmeizhang@hmc.edu.cn
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| Organization name |
Zhejiang Academy of Medical Science
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| Department |
Center for Molecular Medicine
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| Street address |
Tianmushan Road No.182
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310012 |
| Country |
China |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA340459 |
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