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| Status |
Public on Feb 09, 2017 |
| Title |
Enrichment of pluripotent stem cell-derived hepatocyte-like cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The gene expression profiles of differentiating human pluripotent stem cells (hPSCs) were analyzed by DNA microarray. We identified differences and commonalities among six human pluripotent stem cell lines: the hESCs KhES1, KhES2, KhES3, and H1, and the iPSCs 201B7 and 243G1. Analysis of DNA microarray data suggested that hepatocyte-like differentiation of EBs treated with ammonia in Lanford medium.
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| Overall design |
Undifferentiated pluripotent stem cells were prepared using the standard method with MEF-coated dishes (ML) or MEF-conditioned medium method with matrigel-coated dishes (MC). EB were formed by transferring small clumps (200 µm diameter) of hPSCs to a non-adhesive 60-mm dish coated with Lipidure. Suspension cultures were maintained for 5 days. Following EB formation, 1 µL/mL of 10% w/v aqueous ammonia (Wako) was added to fresh Lanford medium. This process was repeated every 2 days for 14 days total.
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| Contributor(s) |
Tomotsune D, Sasaki K |
| Citation(s) |
27632182 |
| Submission date |
Sep 06, 2016 |
| Last update date |
Feb 09, 2017 |
| Contact name |
Daihachiro Tomotsune |
| E-mail(s) |
dtomo@shinshu-u.ac.jp
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| Organization name |
Shinshu University
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| Department |
Institute for Biomedical Sciences
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| Lab |
Department of Biotechnology and Biomedical Engineering
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| Street address |
3-1-1 Asahi
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| City |
Matsumoto |
| State/province |
Nagano |
| ZIP/Postal code |
390-8621 |
| Country |
Japan |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (24)
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| GSM2302929 |
undifferentiated PS cells(ML), H1 |
| GSM2302930 |
undifferentiated PS cells (ML), 201B7 |
| GSM2302931 |
undifferentiated PS cells (ML), 253G1 |
| GSM2302932 |
Fibroblasts, h-Fibroblast |
| GSM2302933 |
differentiated PS cells (EB), KhES1 |
| GSM2302934 |
differentiated PS cells (EB), KhES2 |
| GSM2302935 |
differentiated PS cells (EB), 3-KhES3 |
| GSM2302936 |
differentiated PS cells (EB), H1 |
| GSM2302937 |
differentiated PS cells (EB), 201B7 |
| GSM2302938 |
differentiated PS cells (EB), 253G1 |
| GSM2302939 |
Fibroblasts (EB), Fibro-D |
| GSM2302940 |
differentiated PS cells (Ammonia), 201B7 |
| GSM2302941 |
differentiated PS cells (Ammonia), 253G1 |
| GSM2302942 |
differentiated PS cells (Ammonia), KhES1 |
| GSM2302943 |
differentiated PS cells (Ammonia), KhES2 |
| GSM2302944 |
differentiated PS cells (Ammonia), KhES3 |
| GSM2302945 |
differentiated PS cells (Ammonia), H1 |
| GSM2302946 |
Undifferentiated PS cells (MC), KhES1 |
| GSM2302947 |
Undifferentiated PS cells (MC), KhES2 |
| GSM2302948 |
Undifferentiated PS cells (MC), H1 |
| GSM2302949 |
Undifferentiated PS cells (MC), 253G1 |
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| Relations |
| BioProject |
PRJNA341955 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86453_RAW.tar |
294.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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