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| Status |
Public on Sep 27, 2016 |
| Title |
Activation of Ftz-F1-responsive genes through Ftz/Ftz-F1 dependent enhancers |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
The orphan nuclear receptor Ftz-F1 is expressed in all somatic nuclei in Drosophila embryos but mutations result in a pair-rule phenotype. Previously characterized Ftz-F1 target genes were co-regulated by Ftz, which is expressed in stripes, consistent with the pair-rule phenotype observed for ftz-f1 and ftz mutants. However, attempts to identify new target genes on the basis of Ftz-F1 and Ftz binding sites alone has met with only limited success. To discern the rules for Ftz-F1 target site selection in vivo and to identify additional target genes, a microarray analysis was performed comparing wildtype and ftz-f1 mutant embryos.
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| Overall design |
Drosophila embryos from a cross between w[118] males and FRT germ-line clone ftz-f1 females or FRT germ-line clone w[118] females were hand selected at 3 stages of development (5, 6 and 8) for RNA extraction and hybridization on Affymetrix microarrays.
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The authors state that "Affymetrix CEL files have been lost."
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| |
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| Contributor(s) |
Anderson WR, Pick L |
| Citation(s) |
27723822 |
| Submission date |
Sep 26, 2016 |
| Last update date |
May 04, 2018 |
| Contact name |
William R Anderson |
| E-mail(s) |
wra_3@hotmail.com
|
| Organization name |
University of Maryland
|
| Department |
Entomology
|
| Lab |
Leslie Pick
|
| Street address |
4112 Plant Sciences Building
|
| City |
College Park |
| State/province |
MD |
| ZIP/Postal code |
20742 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA344461 |
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