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| Status |
Public on Jan 01, 2017 |
| Title |
Clonal isolates of PC3 cells overexpressing RWDD4 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We identified RWDD4 as prostate cancer metastases genes by analyzing QTL, transcript profiles and SNP associations with aggressive disease phenotypic traits in transgenic mice and human cohorts
RWDD4 was futher validated as a metastases gene in vitro and in vivo utilizing prostate cancer cell lines overexpressing the gene, and we used microarray to analyze the changes in transcripts
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| Overall design |
Human prostate cancer cell line PC-3 was cloned with etither a control (empty) p-Lenti vector, or a p-Lenti vector containing the RWDD4 gene. Cells were selected using blastocydin followed by propagation of 4 clonal isolates for RNA extraction and hybridization onto Affymetrix array
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| |
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| Contributor(s) |
Winter J, Crawford N |
| Citation(s) |
27916600 |
| Submission date |
Sep 29, 2016 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Jean Winter |
| E-mail(s) |
jean.winter@nih.gov
|
| Organization name |
NIH
|
| Department |
NHGRI
|
| Street address |
50 South Driver
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20814 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE87491 |
Analysis of germline modifiers of aggressive prostate cancer using cross-species variation and systems genetics |
|
| Relations |
| BioProject |
PRJNA344907 |
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