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| Status |
Public on Jul 13, 2017 |
| Title |
ASCL1 mediates neuronal differentiation of primary GBM stem cell cultures upon Notch signalling blockade [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
ASCL1 mediates neuronal differentiation of GBM stem cell (GSC) cultures upon Notch signalling inhibition. We sought to identify gene expression changes that were specific to ASCL1 function. In this dataset, we include RNA-seq data obtained from GSC cultures harbouring wildtype or CRISPR-deletion of ASCL1. We assessed differential gene expression between wildtype and ASCL1-knockout after treatment with gamma-secretase inhibitor for 7 days.
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| Overall design |
Glioblastoma cells with a genetic knockout of ASCL1 is compared to wildtype ASCL1 glioblastoma cells treated with vehicle (DMSO) or gamma-secretase inhibitor (n = 3 biological replicates; total = 12 samples)
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| Contributor(s) |
Park NI, Dirks PB |
| Citation(s) |
28712938 |
| Submission date |
Oct 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter B Dirks |
| Organization name |
The Hospital for Sick Children
|
| Department |
Arthur and Sonia Labatt Brain Tumour Research Centre and Division of Neurosurgery
|
| Street address |
686 Bay St
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA345387 |
| SRA |
SRP090830 |
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