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| Status |
Public on Jul 13, 2017 |
| Title |
Identifying ASCL1 target genes in primary GBM stem cell cultures [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ASCL1 mediates neuronal differentiation of GBM stem cell (GSC) cultures. We sought to identify genomic targets of ASCL1 in primary human GSC cultures. In this dataset, we include ChIP-seq data obtained from GSC cultures harbouring a CRISPR-deletion of ASCL1. We assessed differential ASCL1 binding between control and GSC cultures induced to overexpress ASCL1 after 18 hours of doxycycline treatment.
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| Overall design |
Glioblastoma cells with a genetic knockout of ASCL1 is compared to genetic knockout of ASCL1 overexpressing ASCL1 (n = 3 biological replicates; n = 2 input samples; total = 8 samples)
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| Contributor(s) |
Park NI, Guilhamon P, Lupien M, Dirks PB |
| Citation(s) |
28712938 |
| Submission date |
Oct 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter B Dirks |
| Organization name |
The Hospital for Sick Children
|
| Department |
Arthur and Sonia Labatt Brain Tumour Research Centre and Division of Neurosurgery
|
| Street address |
686 Bay St
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA345388 |
| SRA |
SRP090832 |
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