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| Status |
Public on Nov 04, 2016 |
| Title |
A novel translational control mechanism involving RNA structures within coding sequences |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
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| Summary |
The impact of RNA structures in coding sequences (CDS) within mRNAs is poorly understood. Here we identify a novel and highly conserved mechanism of translational control involving RNA structures within coding sequences and the DEAD-box helicase Dhh1. Using yeast genetics and genome-wide ribosome profiling analyses we show that this mechanism, initially derived from studies of the Brome Mosaic virus RNA genome, extends to yeast and human mRNAs highly enriched in membrane and secreted proteins. All Dhh1-dependent mRNAs, viral and cellular, share key common features. First, they contain long and highly structured CDSs, including a region located around nucleotide 70 after the translation initiation site, second, they are directly bound by Dhh1 with a specific binding distribution and third, complementary experimental approaches suggest that they are activated by Dhh1 at the translation initiation step. Our results show that ribosome translocation is not the only unwinding force of CDS and uncover a novel layer of translational control that involves RNA helicases and RNA folding within CDS providing novel opportunities for regulation of membrane and secretome proteins.
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| Overall design |
Wild-type BY4741 yeast cells or cells expressing genomically His-Tev-ProtA-tagged Dhh1 were crosslinked using UV and analysed by CRAC.
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| Contributor(s) |
Jungfleisch J, Nedialkova DD, Dotu I, Sloan KE, Martinez-Bosch N, Brüning L, Raineri E, Navarro P, Bohnsack MT, Leidel SA, Díez J |
| Citation(s) |
27821408 |
| Submission date |
Oct 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Markus T. Bohnsack |
| E-mail(s) |
markus.bohnsack@med.uni-goettingen.de
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| Organization name |
Goettingen University
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| Department |
Institute for Molecular Biology
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| Street address |
Humboldtallee 23
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| City |
Goettingen |
| ZIP/Postal code |
37073 |
| Country |
Germany |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA348109 |
| SRA |
SRP091450 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE87888_Dhh1_mRNAs.txt.gz |
11.5 Kb |
(ftp)(http) |
TXT |
| GSE87888_RAW.tar |
930.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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