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| Status |
Public on Feb 22, 2019 |
| Title |
Gene expression profiles of microdissected tumor epithelium and stroma from TN breast tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Engaging the immune system promises to be key for optimal cancer therapy, especially in hard-to-treat disease such as triple-negative breast cancer (TNBC). However, the immune microenvironment remains poorly understood. Using gene expression based on laser capture microdissection, we identify three distinct tumor microenvironments associated with CD8+ T cell localization patterns and outcome in TNBC. An immunoreactive microenvironment is defined by granzyme B-positive CD8+ T cell infiltration, a type I interferon signature, tumor expression of PD-L1 and good outcome. In contrast, tumors with an immune-cold microenvironment display restriction of CD8+ T cells to tumor margins, elevated tumor expression of the immunosuppressive marker B7-H4, a signature of desmoplastic stroma and poor outcome. A third distinct immunomodulatory microenvironment associated with poor outcome is enriched for IL-17-producing cells and neutrophils, as well as stroma-restricted localisation of CD8+ T cells. These distinct immune microenvironments have implications for TNBC patient stratification for current and future therapies.
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| Overall design |
38 patient samples, tissue compartments isolated by laser capture microdissection to yield 38 samples of tumor epithelium, 38 samples of tumor stroma; each subjected to gene expression profiling in a common reference design using Agilent whole human genome oligonucleotide arrays.
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| Contributor(s) |
Park M |
| Citation(s) |
30753167 |
| Submission date |
Oct 13, 2016 |
| Last update date |
Feb 22, 2019 |
| Contact name |
Benjamin Haibe-Kains |
| E-mail(s) |
benjamin.haibe.kains@utoronto.ca
|
| Phone |
+14165818626
|
| Organization name |
Princess Margaret Cancer Centre
|
| Department |
Princess Margaret Research
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (76)
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| Relations |
| BioProject |
PRJNA348764 |
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