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| Status |
Public on Mar 21, 2017 |
| Title |
Transcriptomic screen to identify genes regulated by Store-operated calcium entry in Drosophila pupal nervous system |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptional regulation by Store-operated Calcium Entry (SOCE) is well studied in non-excitable cells. However, the role of SOCE has been poorly documented in neuronal cells with more complicated calcium dynamics. Previous reports demonstrated a requirement of neuronal SOCE for Drosophila flight. We identified the early pupal stage to be critical and used RNA-sequencing to identify SOCE mediated gene expression changes in the developing Drosophila pupal nervous system. We down-regulated dStim, the endoplasmic reticular calcium sensor and a principal component of SOCE in the nervous system for a 24h period during pupal development, and compared wild type and knockdown transcriptional profiles, immediately after knockdown as well as after a 36h recovery period. We found that dStim knockdown altered the expression of a number of genes. We also characterized one of the down-regulated genes, Ral for its role in flight. Thus, we identify neuronal SOCE as a mechanism that regulates expression of a number of genes during the development of the pupal nervous system. These genes can be further studied in the context of pupal nervous system development.
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| Overall design |
mRNA sequencing from two biological replicates each of wild type and dStim knockdown pupal brains at two time points - 36h APF (post 24h knockdown) and at 72h APF (Post knockdown and recovery)
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| Contributor(s) |
Hasan G, Richhariya S |
| Citation(s) |
28195208 |
| Submission date |
Oct 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Gaiti Hasan |
| E-mail(s) |
gaiti@ncbs.res.in
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| Organization name |
NCBS, TIFR
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| Street address |
GKVK Campus,
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| City |
Bangalore |
| ZIP/Postal code |
560065 |
| Country |
India |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA350516 |
| SRA |
SRP092054 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89168_36hpupalCNS_WT_dStimKD_CuffDiff.txt.gz |
477.9 Kb |
(ftp)(http) |
TXT |
| GSE89168_36hpupalCNS_WT_dStimKD_EdgeR.txt.gz |
213.1 Kb |
(ftp)(http) |
TXT |
| GSE89168_72hpupalCNS_WT_dStimKD+Recovery_CuffDiff.txt.gz |
476.8 Kb |
(ftp)(http) |
TXT |
| GSE89168_72hpupalCNS_WT_dStimKD+Recovery_EdgeR.txt.gz |
228.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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