|
| Status |
Public on Jul 17, 2017 |
| Title |
A genome-wide CRISPR activation screen identifies B4GALNT2 as an inhibitory host factor for influenza virus infection |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Other
|
| Summary |
Identification of factors to inhibit influenza virus infection
|
| |
|
| Overall design |
CRISPR activation technology was used to identify genes that rendered the cell refractory to infection. The complete sgRNA library (plasmid sample) were packaged into lentivirus and used to transduce A549 cells (transduced sample). In three independent biological replicates, the transduced cells were infected with influenza virus, and the cells that were refractory to infection were collected and the sgRNAs enriched in those populations were sequenced (samples A, B, and C).
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| |
|
| Contributor(s) |
Heaton NS |
| Citation(s) |
28813663 |
| Submission date |
Nov 01, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicholas Heaton |
| E-mail(s) |
nicholas.heaton@duke.edu
|
| Organization name |
Duke University School of Medicine
|
| Department |
Molecular Genetics and Microbiology
|
| Street address |
213 Research Drive, 426 CARL Building
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
|
| Samples (9)
|
| GSM2367503 |
Sequencing of the plasmid sgRNA library_lane 1 |
| GSM2367504 |
Sequencing of the plasmid sgRNA library_lane 2 |
| GSM2367505 |
Sequencing of the transduced sgRNAs pre-selection_lane 1 |
| GSM2367506 |
Sequencing of the transduced sgRNAs pre-selection_lane 2 |
| GSM2367507 |
Sequencing of the transduced sgRNAs post-selection (Screen A)_lane 1 |
| GSM2367508 |
Sequencing of the transduced sgRNAs post-selection (Screen A)_lane 2 |
| GSM2367509 |
Sequencing of the transduced sgRNAs post-selection (Screen B)_lane 1 |
| GSM2367510 |
Sequencing of the transduced sgRNAs post-selection (Screen B)_lane 2 |
| GSM2367511 |
Sequencing of the transduced sgRNAs post-selection (Screen C) |
|
| Relations |
| BioProject |
PRJNA351924 |
| SRA |
SRP092380 |
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