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| Status |
Public on Feb 14, 2017 |
| Title |
SARS-CoV-Encoded Small RNAs Contribute to Infection-Associated Lung Pathology |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Severe acute respiratory syndrome coronavirus (SARS-CoV) causes lethal disease in humans, which is characterized by exacerbated inflammatory response and extensive lung pathology. To address the relevance of small non-coding RNAs in SARS-CoV pathology, we deep sequenced RNAs from the lungs of infected mice and discovered three 18–22 nt small viral RNAs (svRNAs). The three svRNAs were derived from the nsp3 (svRNA-nsp3.1 and -nsp3.2) and N (svRNA-N) genomic regions of SARS-CoV. Biogenesis of CoV svRNAs was RNase III, cell type, and host species independent, but it was dependent on the extent of viral replication. Antagomir-mediated inhibition of svRNA-N significantly reduced in vivo lung pathology and pro-inflammatory cytokine expression. Taken together, these data indicate that svRNAs contribute to SARS-CoV pathogenesis and highlight the potential of svRNA-N antagomirs as antivirals.
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| Overall design |
Strand-specific, single-end, reads were generated for detecting smallRNAs (18 nts or more) in lung (mouse). Three or four replicates were prepared per sample type.
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| Contributor(s) |
Morales L, Oliveros JC, Fernández-Delgado R, tenOever BR, Enjuanes L, Sola I |
| Citation(s) |
28216251 |
| Submission date |
Nov 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Juan Carlos Oliveros |
| Organization name |
CNB, CSIC
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| Street address |
Darwin 3
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| City |
Cantoblanco |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
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| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA355238 |
| SRA |
SRP094035 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE90624_SARS_peaks_read_counts.txt.gz |
525 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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