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| Status |
Public on Nov 01, 2007 |
| Title |
Gene expression profiling of E12.5 wildtype- and Sp3 null hearts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mice lacking the zinc finger transcription factor Specificity protein 3 (Sp3) die prenatally in the C57Bl/6 background. To elucidate the cause of mortality we analyzed the potential role of Sp3 in embryonic heart development. Sp3 null hearts display defective looping at E10.5, and at E14.5 the Sp3 null mutants have developed a range of severe cardiac malformations. In an attempt to position Sp3 in the cardiac developmental hierarchy, we analysed the expression patterns of >15 marker genes in Sp3 null hearts. Expression of Cardiac ankyrin repeat protein (Carp) was downregulated prematurely after E12.5, while expression of the other marker genes was not affected. ChIP analysis revealed that Sp3 is bound to the Carp promoter region in vivo. Microarray analysis indicates that small molecule metabolism and cell-cell interactions are the most significantly affected biological processes in E12.5 Sp3 null myocardium. Since the epicardium showed distension from the myocardium, we studied expression of Wt1, a marker for epicardial cells. Wt1 expression was diminished in epicardium-derived cells in the myocardium of Sp3 null hearts. We conclude that Sp3 is required for normal cardiac development, and suggest that it has a crucial role in myocardial differentiation. (
Keywords: Transcription factors, Sp3, knockout mice, cardiac malformations, E12.5
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| Overall design |
Hearts were dissected from E12.5 wildtype (n=3) and Sp3 knockout (n=3) fetuses. Total RNA was isolated from individual hearts; 5μg was used for labelling and hybridization to 430 2.0 Gene Chips (a total of 6).
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| Contributor(s) |
van Loo P, Mahtab E, Wisse L, Hou J, Grosveld F, Suske G, Philipsen S, Gittenberger-de Groot A |
| Citation(s) |
17923686 |
| Submission date |
Sep 21, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Sjaak Philipsen |
| E-mail(s) |
j.philipsen@erasmusmc.nl
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| Phone |
+31-10-7044282
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| Fax |
+31-10-7044743
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| URL |
http://www.erasmusmc.nl/medical_genetics/
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| Organization name |
Erasmus MC
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| Department |
Cell Biology
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| Street address |
Dr. Molewaterplein 50
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| City |
Rotterdam |
| ZIP/Postal code |
3015 GE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA102663 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9124_RAW.tar |
22.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
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