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| Status |
Public on Jan 01, 2017 |
| Title |
Exogenous miRNA expression profiling in serum of pig and rat fed with different diets |
| Organisms |
Sus scrofa; Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We performed carefully designed and controlled animal feeding studies. We devided 9 adult rats in three group and each group fed with one type of diets in 28 days: rice, potatoes and chow. We fed piglets in triplicate either with cow milk or let them feed from sows milk for 4 weeks followed by 7 weeks of maize diet. Based on the small RNA sequencing data from 9 rat and 6 pig serum samples, we detected no transfer of plant miRNAs into rat blood, or bovine milk sequences into piglet blood.
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| Overall design |
The miRNA profiles in serum of chow-fed, potato-fed and rice-fed rats in 28 days and the miRNA profiles in serum of cow milk and sows milk fed pigs were generated by sequencing, in triplicate, Illumina TruSeq small RNA.
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| Contributor(s) |
Kang W, Bang-Bertelsen C, Holm A, Houben A, Holt Müller A, Thymann T, Pociot F, Estivill X, Friedländer MR |
| Citation(s) |
28062594 |
| Submission date |
Dec 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Marc Riemer Friedländer |
| E-mail(s) |
marc.friedlander@scilifelab.se
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| Organization name |
Stockholm University / SciLifeLab
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| Department |
Department of Molecular Biosciences, The Wenner-Gren Institute
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| Lab |
Lab Friedländer
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| Street address |
Svante Arrhenius väg 20C
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| City |
Stockholm |
| ZIP/Postal code |
10691 |
| Country |
Sweden |
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| Platforms (2) |
| GPL11429 |
Illumina HiSeq 2000 (Sus scrofa) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA358730 |
| SRA |
SRP095622 |
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