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| Status |
Public on Jul 18, 2017 |
| Title |
Distinct Sets Of Genes Representing Overlapping Biological Functions Are Altered By Intrinsic Properties Of Carbon Nanomaterials In Vitro And In Vivo [human] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Six different carbon nanomaterials (CNM) administered by oropharyngeal aspiration to C57BL/6 mice (on 4 consecutive days with 10ug of CNM per day in PBS, sacrificed on 5th day) and to differentiated THP-1 cells in culture (6 hour and 24 hour, with 100ug of CNM /ml of medium)
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| Overall design |
THP-1 cells were grown in 30 ml of RPMI 1640 media, supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin (PEST) antibiotics and 2mM Ultraglutamine. THP-1 cells were differentiated with 50nm PMA (phorbol-12-myristate-13-acetate) and exposed in six-well plates to six different carbon nanomaterials (CNM) by replacing the old media and adding 100 µg of the CNM in 1 ml of fresh complete RPMI and incubated for 6 or 24 hours
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| Contributor(s) |
Kinaret P, Marwah V, Fortino V, Ilves M, Wolff H, Ruokolainen L, Auvinen P, Savolainen K, Alenius H, Greco D |
| Citation(s) |
28380293 |
| Submission date |
Dec 23, 2016 |
| Last update date |
Oct 17, 2017 |
| Contact name |
Dario Greco |
| E-mail(s) |
dario.greco@tuni.fi
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| Organization name |
Tampere University
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| Department |
Faculty of Medicine and Health Technology
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| Lab |
Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
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| Street address |
Arvo ylpön Katu 34
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| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
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| Platforms (1) |
| GPL10332 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (48)
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| This SubSeries is part of SuperSeries: |
| GSE92901 |
Distinct Sets Of Genes Representing Overlapping Biological Functions Are Altered By Intrinsic Properties Of Carbon Nanomaterials In Vitro And In Vivo |
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| Relations |
| BioProject |
PRJNA358737 |
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