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| Status |
Public on Mar 12, 2019 |
| Title |
Transcriptomic analysis of strains evolved for anaerobic xylose utilization [Y223_Y127_Y128] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Engineering microbes with novel metabolic properties is a critical step for production of biofuels and biochemicals. Synthetic biology enables identification and engineering of metabolic pathways into microbes; however, knowledge of how to reroute cellular regulatory signals and metabolic flux remains lacking. Here we used network analysis of multi-omic data to dissect the mechanism of anaerobic xylose fermentation, a trait important for biochemical production from plant lignocellulose. We compared transcriptomic, proteomic, and phosphoproteomic differences across a series of strains evolved to ferment xylose under various conditions.
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| |
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| Overall design |
RNA-seq and transcriptome analysis of three evolved S. cerevisiae strains (Y22-3, Y127, Y128) grown aerobically or anaerobically in rich lab media with glucose, xylose, galactose, or sorbitol. Duplicates were collected on different days.
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| |
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| Contributor(s) |
Myers K, Gasch A |
| Citation(s) |
30856163 |
| Submission date |
Dec 24, 2016 |
| Last update date |
Jun 11, 2019 |
| Contact name |
Kevin S Myers |
| E-mail(s) |
kmyers2@wisc.edu
|
| Organization name |
University of Wisconsin - Madison
|
| Department |
Great Lakes Bioenergy Research Center
|
| Street address |
5127 WEI, 1552 University Ave
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53726 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (32)
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| This SubSeries is part of SuperSeries: |
| GSE92908 |
Transcriptomic analysis of strains evolved for anaerobic xylose utilization |
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| Relations |
| BioProject |
PRJNA358773 |
| SRA |
SRP095642 |
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