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| Status |
Public on Apr 28, 2017 |
| Title |
Identification of the Lgr5+ population in human colorectal samples |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Lgr5+ cell population is the stem cell population that fuels intestinal growth in homeostatic conditions. Here, we have labeled with EGFP the Lgr5 expressing population in patient derived organoids, injected them subcutaneously in mice and disaggregated the tumors to sort the EGFP+ population and characterize its gene expression profile. This Lgr5-EGFP population is more proficient in comparison to the negative in propagating tumors both in vitro (assessed by organoid formation) and in vivo (assessed by Tumor initiation experiments)
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| |
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| Overall design |
Total RNA was collected from the EGFP+ (Lgr5+) population and EGFP- (Lgr5-) population after FACs sorting
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| Contributor(s) |
Stephan-Otto Attolini C, Cortina C, Turon G |
| Citation(s) |
28468934 |
| Submission date |
Dec 28, 2016 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Camille Stephan Otto Attolini |
| Organization name |
IRB Barcelona
|
| Department |
Biostatistics and bioinformatics Unit
|
| Street address |
Baldiri Reixac 10
|
| City |
Barcelona |
| State/province |
Select a State or Province |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE92962 |
A genome editing approach to study cancer stem cells in human tumors |
|
| Relations |
| BioProject |
PRJNA359322 |
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