|
| Status |
Public on Mar 28, 2017 |
| Title |
Gene expression in GSCs with shALKBH5 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Knocking down ALKBH5 in Glioma Stem Cells resulted in an altered gene expression profile
N6-methyl-adenosine (m6A) is the most prevalent internal chemical modification of mRNAs in eukaryotes. In mammals, m6A installed by m6A methyltransferases METTL3 and METTL14 is erased by two members of the AlkB family of nonheme Fe(II)/a-ketoglutarate (a-KG)-dependent dioxygenases, fat-mass and obesity associated protein (FTO) or ALKBH5. ALKBH5 affects nuclear RNA export and metabolism, gene expression and mouse fertility. To date, little is known about the biological significance of m6A in human cancer. We found that ALKBH5 is highly expressed in human glioblastoma stem cells which are resistant to conventional therapy and give rise to glioblastoma recurrence by sustaining long-term tumor growth. Global manipulation of the m6A modification by depleting ALKBH5 resulted in altered gene expression including subsets of genes enriched in "Cell Cycle", "DNA Replication, Recombination, and Repair" and "Cellular Assembly and Organization". Knockdown of ALKBH5 expression in human glioblastoma stem cells significantly reduced their self-renewal ability as a result of inhibition of cell cycle progression. This study demonstrated the important role of m6A modification in human glioblastoma development.
|
| |
|
| Overall design |
GSCs expressing shRNAs for control and ALKBH5 were purimycin-selected for RNA extraction and hybridization on Affymetrix microarrays
|
| |
|
| Contributor(s) |
Zhang S |
| Citation(s) |
28344040 |
| Submission date |
Jan 03, 2017 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Sicong Zhang |
| Organization name |
The Rockefeller University
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA360051 |
Less...
More...