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| Status |
Public on Jan 04, 2017 |
| Title |
Genome-wide DNA Methylation Analysis in Lung Fibroblasts Co-cultured with Silica-exposed Alveolar Macrophages |
| Organism |
Rattus norvegicus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
In the present study, we used MeDIP coupled with next-generation sequencing to profile the whole genome DNA methylation patterns of fibroblast from the co-culture model in vitro. We analyzed the data to identify possible biological pathways for fibroblast differentiating into myofibroblast as well as the interactions of related genes.
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| Overall design |
The co-culture was established of AMs and fibroblasts. Seeding fibroblasts into six-well plates and incubated for one or two days until approximately 60% confluence was achieved. AMs were seeding into the inserts and placed the insert into the six-well dish and then exposed to 100µg/ml SiO2 for 0-, 24-, and 48 hours
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| Contributor(s) |
Li J, Hao C, Hou J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 04, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Juan Li |
| E-mail(s) |
xiaoen222000@yahoo.com.cn
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| Organization name |
Zhengzhou University
|
| Department |
college of public health
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| Street address |
kexue road
|
| City |
Zhengzhou city |
| State/province |
Henan |
| ZIP/Postal code |
475001 |
| Country |
China |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA360081 |
| SRA |
SRP096010 |
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