|
| Status |
Public on Jan 23, 2018 |
| Title |
BL-Hi-C is an efficient and sensitive approach for capturing structural and regulatory chromatin interactions |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We propose a novel in situ Hi-C method named Bridge-Linker-Hi-C (BL-Hi-C) for structural and regulatory chromatin interactions capture by restriction enzyme targeting and two-step proximity ligation. It improves the sensitivity and specificity for active chromatin loop detection and can reveal a better enhancer-promoter regulatory architecture than conventional method at a lower sequencing depth and with a simpler protocol.
|
| |
|
| Overall design |
BL-Hi-C was performed in K562 cell for 13 samples: 5 samples for enrichment analysis; 4 samples for enzyme digestion validation and 4 samples for proximity ligation validation.
|
| |
|
| Contributor(s) |
Liang Z, Zhang MQ, Chen Y |
| Citation(s) |
29158486 |
| Submission date |
Jan 23, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Zhengyu LIANG |
| E-mail(s) |
zyliang.cn@gmail.com
|
| Organization name |
Southern University of Science and Technology
|
| Department |
Department of Biology
|
| Lab |
Zhengyu Liang
|
| Street address |
1088 Xueyuan Avenue
|
| City |
Shenzhen |
| State/province |
Guangzhou |
| ZIP/Postal code |
518055 |
| Country |
China |
| |
|
| Platforms (2) |
|
| Samples (19)
|
|
| Relations |
| BioProject |
PRJNA362848 |
| SRA |
SRP097607 |
Less...
More...