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| Status |
Public on Mar 31, 2018 |
| Title |
Gene expression profiles of RALD iPSCs cultured with or without bFGF |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We investigated roles of KRAS on stemness maintenance in the context of induced pluripotent stem cells (iPSCs) using isogenic KRAS mutant (G13C/WT) and wild-type (WT/WT) iPSCs from the same RAS-associated autoimmune lymphoproliferative syndrome-like disease (RALD) patients. Microarray analysis was conducted to compare gene expression profiles of WT/WT and G13C/WT iPS cells cultured with or without bFGF. Expression of stemness markers including POU5F1 and NANOG, which is indicative of an undifferentiated state, was highly maintained in G13C/WT iPSCs, but not in WT/WT iPSCs, under bFGF depletion, indicating enhanced self-renewal of iPSCs by the oncogenic KRAS.
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| Overall design |
To investigate the changes of undifferentiation and differentiation markers between KRAS mutant and wild-type iPSCs under the presence or absence of bFGF, the iPSCs were cultured with or without bFGF for 5 days. Two clones were used for each genotype, resulting in total 8 conditions.
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| Contributor(s) |
Kubara K, Yamazaki K, Ishihara Y, Lin HT, Ito M, Tsukahara K, Takagi M, Otsu M |
| Citation(s) |
29983389 |
| Submission date |
Jan 27, 2017 |
| Last update date |
Jan 10, 2019 |
| Contact name |
Kenji Kubara |
| E-mail(s) |
k-kubara@hhc.eisai.co.jp
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| Organization name |
Eisai Co., Ltd.
|
| Department |
Tsukuba Research Laboratories
|
| Street address |
Tokodai 5-1-3
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
300-2635 |
| Country |
Japan |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA369072 |
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