 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 09, 2017 |
| Title |
RNASeq of juvenile keratinocytes, melanocytes, fibroblasts and whole skin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used whole transcriptome sequencing (RNA-Seq) to assess differential gene expression of normal human melanocytes with respect to keratinocytes, fibroblasts and whole skin. We have identified a number of genes and pathways, which are characteristic or unique for MC compared to KC and FB. We also demonstrated the extent of the difference between genes detectable in MC culture and the whole skin. The present results reveal melanocytes as highly proliferative cells with stem cell-like properties.
|
| |
|
| Overall design |
From each of the healthy juvnile skin tissue samples a whole skin sample was taken and three skin cell types (keratinocytes, melanocytes and fibroblasts) were harvested and cultivated. We chose 12 total RNA samples, extracted from 4 keratinocytes (Kc), 4 melanocytes (Mc), 2 fibroblasts (FB) and 2 whole skin samples for library preparation.
|
| |
|
| Contributor(s) |
Reeman P |
| Citation(s) |
28267805 |
| Submission date |
Feb 08, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Paula Reemann |
| Organization name |
University of Tartu
|
| Department |
Institute of Biomedicine and Translational Medicine, Department of Physiology
|
| Street address |
Ravila 19
|
| City |
Tartu |
| ZIP/Postal code |
50411 |
| Country |
Estonia |
| |
|
| Platforms (1) |
| GPL16288 |
AB 5500xl Genetic Analyzer (Homo sapiens) |
|
| Samples (12)
|
| GSM2480392 |
juvenile skin fibroblasts, patient's code C026 |
| GSM2480393 |
juvenile skin melanocytes, patient's code C002 |
| GSM2480394 |
juvenile skin melanocytes, patient's code C026 |
| GSM2480395 |
juvenile skin melanocytes, patient's code C022 |
| GSM2480396 |
juvenile skin keratinocytes, patient's code C024 |
| GSM2480397 |
juvenile skin biopsy, patient's code C011 |
| GSM2480398 |
juvenile skin biopsy, patient's code C014 |
| GSM2480399 |
juvenile skin fibroblasts, patient's code C025 |
| GSM2480400 |
juvenile skin keratinocytes, patient's code C025 |
| GSM2480401 |
juvenile skin keratinocytes, patient's code C030 |
| GSM2480402 |
juvenile skin keratinocytes, patient's code C032 |
| GSM2480403 |
juvenile skin melanocytes, patient's code C032 |
|
| Relations |
| BioProject |
PRJNA371742 |
| SRA |
SRP099048 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE94655_RAW.tar |
165.8 Mb |
(http)(custom) |
TAR (of FPKM_TRACKING, GTF) |
| GSE94655_htseq_count_table.txt.gz |
379.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...