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| Status |
Public on Oct 16, 2017 |
| Title |
Biological sex influences gene expressions in cardiac myocytes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose:Heart disease is the number one killer of men and women, but not much is known about baseline differences in the heart between males and females
Method: Adult rat ventricular myocytes (ARVMs) were isolated from male and female rats and then RNA was isolated and RNA sequencing was performed.
Results: We identified ~ 600 transcripts that were differentially expressed in cardiac myocytes from either sex. We also observed that enriched pathways from this data set were sexually dimorphic
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| Overall design |
ARVMs were isolated, plated for 45 minutes and then frozen with liquid nitrogen. We had at least 5 biological replicates for each sex; n=6 males and n=5 females
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| |
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| Contributor(s) |
Trexler C, Leinwand L |
| Citation(s) |
29030402 |
| Submission date |
Feb 22, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Leslie Leinwand |
| E-mail(s) |
leslie.leinwand@colorado.edu
|
| Phone |
3034927606
|
| Organization name |
University of Colorado at Boulder
|
| Department |
MCDB
|
| Lab |
Leinwand
|
| Street address |
596 UCB
|
| City |
Boulder |
| State/province |
CO |
| ZIP/Postal code |
80309 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA376550 |
| SRA |
SRP100621 |
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