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| Status |
Public on Sep 04, 2018 |
| Title |
Transcriptional profiling of irritant contact dermatitis (ICD) in a mouse model identifies specific patterns of gene expression and immune-regulation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Irritant contact dermatitis (ICD) is a non-immunological, cutaneous inflammatory response to a variety of triggers that requires no sensitization and accounts for up to 80% of occupational dermatitis cases. Numerous inflammatory cytokines play critical roles in the mechanism and severity of ICD, one being interleukin-6 (IL-6). Therefore, to investigate the influence of this pleotropic cytokine transcriptome analysis (MiSeq) was employed examining irritant-exposed and control skin samples from C57 and IL-6KO mice to gain a better understanding of the immunological mediators of ICD and what role IL-6 plays. Overall, this study offers new insight into the pathogenesis of ICD, indicates new mediators/markers that may influence the variability of responses to irritants and potential targets for therapeutic manipulation.
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| Overall design |
Examination of non-treated skin (C57 and IL-6KO, control samples), and 7 day BKC treated skin (C57-BKC and KO-BKC) in C57 and IL-6KO mouse skin. There are 3 replicates per group for a total of 12 samples and MiSeq analysis was performed.
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| Contributor(s) |
Luckett-Chastain L, Gallucci R |
| Citation(s) |
30171875 |
| Submission date |
Feb 23, 2017 |
| Last update date |
Jan 27, 2019 |
| Contact name |
Lerin Luckett-Chastain |
| E-mail(s) |
jolerin-chastain@ouhsc.edu
|
| Phone |
405-271-6593
|
| Organization name |
OUHSC
|
| Department |
Pharmaceutical Science
|
| Lab |
Gallucci
|
| Street address |
1110 N. Stonewall
|
| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73117 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA376616 |
| SRA |
SRP100651 |
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