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| Status |
Public on Jul 03, 2018 |
| Title |
Targeting oncogenic β-catenin/CBP activity for the treatment of head and neck squamous cell carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Head and neck squamous cell carcinoma (HNSCC) presents primarily as oral squamous cell carcinoma (OSCC), an aggressive malignancy characterized by heterogeneity, locoregional metastases and resistance to existing treatments. Although a number of molecular alterations associated with OSCC have been identified, they have had limited impact on clinical management. A frequent feature of OSCC is the inappropriate activation of nuclear β-catenin. Here, we provide a comprehensive characterization of the effects of blocking the interaction between nuclear β-catenin and the cAMP-responsive element binding (CREB)-binding protein (CBP) using the small molecule inhibitor ICG-001. We demonstrate that ICG-001 inhibits cell proliferation and aggressive OSCC cell phenotypes in cellular, zebrafish and murine models, and that its inhibition-associated transcriptional signature tracks with advanced tumor grade and poor survival in human patients. Our results suggest that targeting the β-catenin/CBP interaction in the nucleus represents a new and effective therapy for OSCC.
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| Overall design |
Microarray gene expression profiling pertaining to ICG-001 or DMSO (control) treatment in HSC-3 and CAL27 cells, and siCTNNB1 or scrambled siRNA control in HSC-3 cells ; n=3 per treatment group, per cell line).
Note: the preprocessed data were generated from two normalizations: 1) samples GSM2522361-GSM2522372, and 2) samples GSM2522373-GSM2522378, plus six other arrays that are not included in this Series. The preprocessed data for samples GSM2522373-GSM2522378 therefore cannot be reproduced entirely from this Series alone.
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| Contributor(s) |
Kartha V, Kukuruzinska M |
| Citation(s) |
30029671 |
| Submission date |
Mar 06, 2017 |
| Last update date |
Jun 25, 2019 |
| Contact name |
Boston University Microarray and Sequencing Resource |
| E-mail(s) |
msrdata@bu.edu
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| Organization name |
Boston University
|
| Department |
Microarray and Sequencing Resource
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| Street address |
72 East Concord Street, E631
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL17930 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [HuGene20stv1_Hs_ENTREZG_17.0.0] |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA378165 |
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