|
| Status |
Public on Jun 07, 2017 |
| Title |
Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23 |
| Platform organism |
Macaca mulatta |
| Sample organisms |
Macaca mulatta; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Third-party reanalysis
|
| Summary |
Primordial germ cells (PGCs) are the earliest embryonic progenitors in the germline. Correct formation of PGCs is critical to reproductive health as an adult. Recent work has shown that primate PGCs can be differentiated from pluripotent stem cells; however, a bioassay that supports their identity as transplantable germ cells has not been reported. Here, we adopted a xenotransplantation assay by transplanting single-cell suspensions of human and nonhuman primate embryonic Macaca mulatta (rhesus macaque) testes containing PGCs into the seminiferous tubules of adult busulfan-treated nude mice. We discovered that both human and nonhuman primate embryonic testis are xenotransplantable, generating colonies while not generating tumors. Taken together, this work provides two critical references (molecular and functional) for defining transplantable primate PGCs. These results provide a blueprint for differentiating pluripotent stem cells to transplantable PGC-like cells in a species that is amenable to transplantation and fertility studies.
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| |
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| Overall design |
10 mRNAseq for Rhesus Macaque
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| |
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| Contributor(s) |
Di C, Liu W |
| Citation(s) |
28579394 |
| Submission date |
Mar 06, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Wanlu Liu |
| Organization name |
Zhejiang University
|
| Department |
Zhejiang University - University of Edinburgh Institute
|
| Lab |
Wanlu Liu
|
| Street address |
718 East Haizhou Rd.,
|
| City |
Haining |
| State/province |
Zhejiang |
| ZIP/Postal code |
314400 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL14954 |
Illumina HiSeq 2000 (Macaca mulatta) |
|
| Samples (10)
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|
| Relations |
| Reanalysis of |
GSM1466233 |
| Reanalysis of |
GSM1466234 |
| Reanalysis of |
GSM1574595 |
| Reanalysis of |
GSM1574596 |
| BioProject |
PRJNA378239 |
| SRA |
SRP101442 |
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