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| Status |
Public on Oct 24, 2017 |
| Title |
Effect of increased insulin signaling on third instar larval fat body gene expression in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We characterized constitutively active insulin receptor (InR)-dependent gene expression in the Drosophila fat body. Transient activation of InR was elicited via heat shock and RNA-seq was used to identify potential target genes.
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| Overall design |
Drosophila were reared on control diets until the third instar stage. Control (hs-GAL4 x y1w1118) offspring were compared with InRCA (hs-GAL4 x UAS-InR.A1325D). Fat bodies were isolated 6 hours after a heat shock, and RNA was extracted to determine the effects of transiently increased InR activation on gene expression using Illumina RNA-seq.
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| Contributor(s) |
Musselman L, Baranski T |
| Citation(s) |
29084810 |
| Submission date |
Mar 17, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Laura Palanker Musselman |
| E-mail(s) |
palankl@hotmail.com
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| Organization name |
Binghamton University
|
| Department |
Biological Sciences
|
| Lab |
S3 room 210
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| Street address |
4400 Vestal Parkway East
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| City |
Binghamton |
| State/province |
New York |
| ZIP/Postal code |
13902 |
| Country |
USA |
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| Platforms (1) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA379588 |
| SRA |
SRP102078 |
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