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| Status |
Public on Jul 30, 2018 |
| Title |
Expression data of renal cancer cell treated with Oncolytic virus VSVd51 and Fumaric acid esters such as Dimethyl fumarate |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We propose a strategy to boost the therapeutic efficacy of Oncolytic therapy by combining it with fumaric acid ester such as Dimethyl fumarate (DMF)
The mechanism of action was examined by microarray analysis using the Affymetrix Human PrimeView Array.
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| Overall design |
786-0 cells were plated in 6-well dishes and allowed to adhere overnight. The next day, cells were pretreated for 4hours with DMF, DEF, DEM and DMM or the vehicle. Following pre-treatment, the cells were infected with VSVĪ51 at an MOI of 0.01 or left uninfected. Twenty-four hours post infection, RNA was collected using an RNA-easy kit . Biological triplicates were subsequently pooled and RNA quality was measured using Agilent 2100 Bioanalyzer (Agilent Technologies) before hybridization. Hybridized to Affymetrix Human PrimeView Array . Microarray data was processed using Transcriptome Analysis Console (TAC) 3.0 under default parameters of Gene Level Differential Expression Analysis. Fold change in gene expression was calculated for each gene in relation to uninfected, untreated control.
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| Contributor(s) |
Selman M, Diallo J |
| Citation(s) |
29367345 |
| Submission date |
Apr 03, 2017 |
| Last update date |
Aug 23, 2018 |
| Contact name |
Mohammed Selman |
| E-mail(s) |
mohammed.selman@gmail.com
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| Organization name |
Ottawa Hospital
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| Department |
Cancer Center
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| Lab |
Diallo Lab
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| Street address |
501 Smyth Road
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| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H8L6 |
| Country |
Canada |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (12)
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| GSM2561706 |
786-0, drug treated_DEF |
| GSM2561707 |
786-0, drug treated_DMM |
| GSM2561708 |
786-0, drug treated_DMF |
| GSM2561709 |
786-0, infected, rep1 |
| GSM2561710 |
786-0, infected, rep2 |
| GSM2561711 |
786-0, drug treated and infected_DEM |
| GSM2561712 |
786-0, drug treated and infected_DEF |
| GSM2561713 |
786-0, drug treated and infected_DMM |
| GSM2561714 |
786-0, drug treated and infected_DMF |
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| Relations |
| BioProject |
PRJNA381409 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE97328_RAW.tar |
25.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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