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| Status |
Public on Jan 01, 2019 |
| Title |
MMSET I acts as an oncoprotein and regulates GLO1 expression in t(4;14) multiple myeloma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Multiple myeloma (MM) is characterized by recurrent chromosomal translocations. The translocation t(4;14)(p16;q32) is one of the most common translocation in MMs, affecting 15% of patients, and is associated with very poor prognosis. The histone methyltransferase (HMTase) MMSET is universally overexpressed in t(4;14) MM as a result of the t(4;14) translocation. MMSET is capable of producing 3 major isoforms, the full length MMSET II, short isoforms REIIBP and MMSET I. MMSET II has been suggested to play an important tumorigenic role in t(4;14) MM, but little is yet known about whether and how the MMSET short isoforms contribute to MM tumorigenesis. The aim of this study is to characterize MMSET I roles and determine its downstream targets in t(4;14) MM. In t(4;14) MM cells MMSET I knockdown with shRNAs induced cell apoptosis, reduced colony formation and inhibited tumorigenicity in vivo. We also found MMSET I knockdown decreased GLO1 expression, and ectopic MMSET I increased GLO1 expression, suggesting that MMSET I is an upstream regulator of GLO1. Further analysis indicated that MMSET I bound to GLO1 promoter region and depended on its C-terminus to regulate GLO1 expression. Our preliminary data suggested that MMSET I is an oncoprotein and could regulate GLO1 expression in t(4;14) multiple myeloma cells.
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| Overall design |
KMS11 cells were treated with shRNAs for 72 h. Total RNA was extracted by using the Qiagen RNeasy Mini kit (Germany). Gene expression was performed using the GeneChip™ Human Gene 2.0 ST Array (Affymetrix) following the manufacturer’s instructions. Data analysis was performed using GeneSpring software from Agilent Technologies.
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| Contributor(s) |
Yuan CJ, Zhigang X, Joo CW |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 02, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Zhigang Xie |
| E-mail(s) |
xiezhigang@nus.edu.sg
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| Organization name |
National University of Singapore
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| Department |
CSI Singapore
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| Street address |
14 Medical Drive, CeLS Building #02-07
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| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA385086 |
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