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| Status |
Public on Jun 19, 2017 |
| Title |
Human umbilical cordderived mesenchymal stem cells (UCMSCs) induce a global transcriptional reprogramming in cisplatindamaged human renal proximal tubular epithelial cells (RPTECs). |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To gain insights into the genes involved in the protective effect of human umbilical cordderived mesenchymal stem cells (UCMSCs) on cisplatininjured human renal proximal tubular epithelial cells (RPTECs), we performed a genomewide differential gene expression analysis by Ion AmpliSeqTM Transcriptome sequencing that targets more than 20,000 human genes. Our data indicated that genes involved in the pathways related to mitochondria and energy homeostasis were strongly upregulated by stem cell treatment in respect to cells treated with cisplatin alone, possibly representing a de novo regenerative response induced by UCMSCs.
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| Overall design |
Gene expression profiles of three RPTECs samples (Control, Cispl, and Cispl+UCMSCs) were generated by Ion AmpliSeq Transcriptome sequencing, in triplicate, using Ion Proton Sequencer.
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| Contributor(s) |
Perico L, Morigi M, Rota C, Breno M, Mele C, Noris M, Introna M, Capelli C, Longaretti L, Rottoli D, Conti S, Remuzzi G, Benigni A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 05, 2017 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Marina Morigi |
| Organization name |
IRCCS – Istituto di Ricerche Farmacologiche Mario Negri, Bergamo, Italy
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| Department |
Molecular Medicine
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| Lab |
Cell Biology and Regenerative Medicine
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| Street address |
Parco Scientifico Tecnologico Kilometro Rosso, via Stezzano, 87
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| City |
Bergamo |
| ZIP/Postal code |
24126 |
| Country |
Italy |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA385620 |
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