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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 31, 2017 |
| Title |
The immunosuppressive effect of the tick salivary protein, Salp15, is persistent and has long-term effects in a murine model of hematopoietic transplant |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Salp15, a salivary protein of Ixodes ticks, inhibits the activation of naïve CD4 T cells. Treatment with Salp15 results in immunomodulation in different murine models in which these cells participate. The fate of the CD4 T cells activated in the presence of the immunosuppressor or its long-term effects on these cells are however, unknown. We now show that Salp15 binding to CD4 is persistent and induces a long-lasting immunomodulatory effect. The activity of Salp15 results in sustained diminished antibody production against specific and unrelated antigens. Transcriptionally, the salivary protein provokes a sharp acute effect that includes known activation factors, such as Il2, Cd44, or Il2ra, and that fades over time. The long-term effects exerted by Salp15 do not involve the induction of either anergy traits nor increased populations of regulatory T cells. Similarly, the treatment with the immunomodulatory protein does not result in B cell anergy or the generation of myeloid suppressor cells. However, the immunomodulatory protein induces the increased expression of the ectoenzyme, CD73, in regulatory T cells. Our results suggest that the specific regulation of CD73, a known modulator of adenosine levels, by Salp15 results in long-term cross-antigenic immunomodulatory effects.
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| Overall design |
Genome-wide changes in gene Expression in mouse CD4 T cells activated with anti-CD3/CD28 in the presence of 25 ug/mL of the tick salivary protein, Salp15 or its inactive control (Salp15deltaP11) were generated by RNAseq.
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| Contributor(s) |
Anguita J, Lavin JL |
| Citation(s) |
28878331 |
| Submission date |
May 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jose Luis Lavin |
| E-mail(s) |
joluito@gmail.com
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| Organization name |
CIC bioGUNE
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| Street address |
Parque Tecnológico de Bizkaia Building 502, Floor 0
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| City |
Derio |
| State/province |
Bizkaia |
| ZIP/Postal code |
48160 |
| Country |
Spain |
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| Platforms (1) |
| GPL16173 |
Illumina HiScanSQ (Mus musculus) |
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| Samples (15)
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| GSM2610357 |
Purified splenic CD4 T cells number 1 unstimulated |
| GSM2610358 |
Purified splenic CD4 T cells number 2 unstimulated |
| GSM2610359 |
Purified splenic CD4 T cells number 3 unstimulated |
| GSM2610360 |
Purified splenic CD4 T cells number 1 activated with anti-CD3/CD28 + Salp15deltaP11 day 2 |
| GSM2610361 |
Purified splenic CD4 T cells number 2 activated with anti-CD3/CD28 + Salp15deltaP11 day 2 |
| GSM2610362 |
Purified splenic CD4 T cells number 3 activated with anti-CD3/CD28 + Salp15deltaP11 day 2 |
| GSM2610363 |
Purified splenic CD4 T cells number 1 activated with anti-CD3/CD28 + Salp15 day 2 |
| GSM2610364 |
Purified splenic CD4 T cells number 2 activated with anti-CD3/CD28 + Salp15 day 2 |
| GSM2610365 |
Purified splenic CD4 T cells number 3 activated with anti-CD3/CD28 + Salp15 day 2 |
| GSM2610366 |
Purified splenic CD4 T cells number 1 activated with anti-CD3/CD28 + Salp15deltaP11 day 4 |
| GSM2610367 |
Purified splenic CD4 T cells number 2 activated with anti-CD3/CD28 + Salp15deltaP11 day 4 |
| GSM2610368 |
Purified splenic CD4 T cells number 3 activated with anti-CD3/CD28 + Salp15deltaP11 day 4 |
| GSM2610369 |
Purified splenic CD4 T cells number 1 activated with anti-CD3/CD28 + Salp15 day 4 |
| GSM2610370 |
Purified splenic CD4 T cells number 2 activated with anti-CD3/CD28 + Salp15 day 4 |
| GSM2610371 |
Purified splenic CD4 T cells number 3 activated with anti-CD3/CD28 + Salp15 day 4 |
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| Relations |
| BioProject |
PRJNA385950 |
| SRA |
SRP106692 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE98700_2sc_vs_2s15.tsv.gz |
218.3 Kb |
(ftp)(http) |
TSV |
| GSE98700_4sc_vs_4s15.tsv.gz |
218.7 Kb |
(ftp)(http) |
TSV |
| GSE98700_C_vs_2sc.tsv.gz |
219.2 Kb |
(ftp)(http) |
TSV |
| GSE98700_C_vs_4sc.tsv.gz |
219.6 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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