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| Status |
Public on Apr 24, 2018 |
| Title |
Histone H3.1K27 methylation promotes NHEJ by antagonizing FANCD2 on Chromatin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goals of this project are to study the transcriptome profiling (RNA-seq) of human fribroblast cells undergoing H3K27me3 decrease by histone mutants incorporation (H3.1K27A/M vs H3.1wt) or shRNA-mediated knockdown of specific histone H3K27me3 methyl-transferase EZH2 (shEZH2 vs shNC)
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| Overall design |
mRNA profiles of human primary fibroblast stable cell lines with overexpression of H3.1WT, H3.1K27A or H3.1K27M or shRNA-mediated knock-down of EZH2 or negative control (NC) were generated by deep sequencing, in triplicate, using Illumina next-generation sequencing.
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| Contributor(s) |
Sun F, Zhang Y, Chang J, Sun J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 10, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jin Sun |
| E-mail(s) |
sunjin@tongji.edu.cn
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| Organization name |
Tongji University
|
| Department |
life science and technology
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| Lab |
Jiang
|
| Street address |
siping1239
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| City |
shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA386162 |
| SRA |
SRP106792 |
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