|
| Status |
Public on Feb 15, 2018 |
| Title |
RNA-seq of S2 cells after GFP (control), dKDM4A, or HP1a RNAi |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Analysis of transcriptional changes from genes and repetitive elements after dKDM4A and HP1a RNAi of S2 cells
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| |
|
| Overall design |
After 5 days of RNAi, total RNA was harvested using Trizol reagent from 5 x 106 S2 cells, and mRNA was purified using oligo-dT-conjugated magnetic beads. Library construction was conducted using TruSeq RNA sample preparation kits (Illumina). All sequencing data were obtained from the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley.
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| |
|
| Contributor(s) |
Colmenares SU, Karpen GH |
| Citation(s) |
28743002 |
| Submission date |
May 17, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
serafin colmenares |
| E-mail(s) |
sucolmenares@lbl.gov
|
| Organization name |
lawrence berkeley national lab
|
| Street address |
1 cyclotron road MS977R180A
|
| City |
berkeley |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (6)
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|
| Relations |
| BioProject |
PRJNA387027 |
| SRA |
SRP107213 |
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