|
| Status |
Public on Nov 01, 2017 |
| Title |
Glass promotes the differentiation of both neuronal and non-neuronal cells in the Drosophila eye |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Multiple cell types can be specified from a single pool of progenitors through the combinatorial activity of transcriptional regulators, which activate distinct developmental programs to establish different cell fates. The zinc finger transcription factor Glass is required
for neuronal progenitors in the Drosophila eye imaginal disc to acquire a photoreceptor identity. Glass is also expressed in non-neuronal cone and pigment cells, but its role in these cells is unknown. To examine how Glass activity is affected by the cellular context, we
misexpressed it in different tissues. When expressed in neuroblasts of the larval brain or in epithelial cells of the wing disc, Glass activated both a common core set of target genes and distinct gene sets specific to each tissue. In addition to photoreceptor-specific genes, Glass
induced markers of cone and pigment cells. Cell type-specific glass mutations generated in cone or pigment cells using somatic CRISPR revealed autonomous developmental defects, and expressing Glass specifically in these cells partially rescued glass mutant phenotypes. Glass thus
acts in both neuronal and non-neuronal cells to promote their differentiation into functional components of the eye, suggesting that it is a determinant of organ identity.
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| Overall design |
6 samples in triplicate, 2 experimental (Glass misexpression (Gl ME) in the wing and Gl ME in the brain), 4 controls (wild-type (WT) eye discs, WT wing discs and WT brains and gl60j brains)
Drosophila melanogaster imaginal discs were isolated from Wandering 3rd Instar larvae (W3L) raised at 25C. Gl ME= Glass Misexpression, WT= wild-type. UAS-gl was expressed with tubulin (tub)-GAL4 in clones generated in the wing using Ultrabithorax-Flippase (Ubx-FLP).
The inscuteable (insc)-GAL4 driver was used to misexpress Gl in the larval brain in a gl60j background.
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| Contributor(s) |
Morrison CA, Chen H, Brown S, Treisman JE |
| Citation(s) |
29324767 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F31 EY025129 |
Identifying the transcriptional core logic that determines photoreceptor cell specification |
NEW YORK UNIVERSITY SCHOOL OF MEDICINE |
Carolyn Arlene Morrison |
| R21 EY024826 |
Photoreceptor cell fate specification by the Glass transcription factor |
NEW YORK UNIVERSITY SCHOOL OF MEDICINE |
Jessica E Treisman |
| R01 EY013777 |
Pattern Formation in the Drosophila Eye Disc |
NEW YORK UNIVERSITY SCHOOL OF MEDICINE |
Jessica E Treisman |
|
| Submission date |
May 25, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Carolyn Morrison |
| E-mail(s) |
cam849@nyumc.org
|
| Phone |
212-263-1031
|
| Organization name |
NYU School of Medicine
|
| Department |
Skirball Institute
|
| Lab |
Treisman
|
| Street address |
540 First Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
11746 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA388078 |
| SRA |
SRP108033 |
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