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Status |
Public on Sep 06, 2017 |
Title |
Expression data from a human lung metastatic cell line treated with siPGC1alpha or siControl |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
To understand global expression changes in a knockdown of PGC1alpha (siPGC1alpha) vs control (siControl) in a lung metastatic cell line (4175) Metabolic adaptations play a key role in fuelling tumour growth. However, less is known regarding the metabolic changes that promote cancer progression to metastatic disease. Herein, we reveal that PGC-1a expression and activity are differentially regulated depending on breast cancer metastatic sites. Breast cancer cells that preferentially metastasize to lung display striking upregulation of PGC-1a expression. PGC-1a promotes breast cancer cell migration and invasion in vitro and augments lung metastasis in vivo. MDA-MB-231 lung variant (4175) cells were treated with a short interfering RNA (siRNA) for human PGC-1a (PPARG1A) or control siRNA for 48hrs 24 hours post-plating cells.
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Overall design |
MDA-MB-231 lung variant (4175) cells were treated with a short interfering RNA (siRNA) for human PGC-1a (PPARG1A) or control siRNA for 48hrs 24 hours post-plating cells.
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Contributor(s) |
Andrzjewski S, Johnson RM, Tabariès S, Annis MG |
Citation(s) |
28988825 |
Submission date |
Jun 01, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Julie St-Pierre |
E-mail(s) |
julie.st-pierre@mcgill.ca
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Organization name |
McGill University
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Department |
Department of Biochemistry
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Street address |
3655 Promenade Sir William Osler
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City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H3G 1Y6 |
Country |
Canada |
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Platforms (1) |
GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE99557 |
Expression data from a lung metastatic cell line or metastatic explants in mouse and human |
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Relations |
BioProject |
PRJNA388962 |