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| Status |
Public on Aug 15, 2018 |
| Title |
MDA-MB-231 gene expression upon IRE1 RNase inhibition with MKC-8866 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The IRE1 Rnase domain has been implicated in the pathology of triple negative breast cancer (TNBC), a disease with limited treatment options. The IRE1 Rnase mediates it's effects on the transcriptome via activation of the trancription factor XBP1s and via direct cleavage of mRNA through a process called RIDD. The processes through which the RNase domain contributes to TNBC is not fully understood.
We used a novel small molecule inhibitor of the IRE1 Rnase to find novel targets of IRE1 in TNBC, in an effort to elucidate how it contributes to the disease.
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| Overall design |
A TNBC cell line MDA-MB 231 was treated with the IRE1 Rnase inhibitor for 4, and 24h in normoxic and hypoxic (1% O2) conditions. We aimed to determine effects of inhibiting IRE1 on an early and a later timepoint to have a broader uderstand how IRE1 Rnase effects cells. Recent reports suggest that XBP1s plays a role in controlling the hypoxic stress response in TNBC cells. To find novel hypoxia-specific functions for IRE1 we inhibited the RNase under hypoxic condditions.
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| Contributor(s) |
McGrath E, Logue S, Samali A |
| Citation(s) |
30111846 |
| Submission date |
Jun 07, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Afshin Samali |
| E-mail(s) |
afshin.samali@nuigalway.ie
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| Organization name |
Apoptosis Research Centre
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| Department |
Biomedical Sciences
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| Lab |
NUI Galway
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| Street address |
Newcastle Road, Dangan
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| City |
Galway |
| ZIP/Postal code |
H91 TK33 |
| Country |
Ireland |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| GSM2651978 |
MDA-MB-231, DMSO treated, 4 hours, biological rep1 |
| GSM2651979 |
MDA-MB-231, DMSO treated, 4 hours, biological rep2 |
| GSM2651980 |
MDA-MB-231, DMSO treated, 4 hours, biological rep3 |
| GSM2651981 |
MDA-MB-231,MKC-8866 treated, 4 hours, biological rep1 |
| GSM2651982 |
MDA-MB-231,MKC-8866 treated, 4 hours, biological rep2 |
| GSM2651983 |
MDA-MB-231,MKC-8866 treated, 4 hours, biological rep3 |
| GSM2651984 |
MDA-MB-231,MKC-8866 treated, 24 hours, biological rep1 |
| GSM2651985 |
MDA-MB-231,MKC-8866 treated, 24 hours, biological rep2 |
| GSM2651986 |
MDA-MB-231,MKC-8866 treated, 24 hours, biological rep3 |
| GSM2651987 |
MDA-MB-231, DMSO treated, 1% O2, 4 hours, biological rep1 |
| GSM2651988 |
MDA-MB-231, DMSO treated, 1% O2, 4 hours, biological rep2 |
| GSM2651989 |
MDA-MB-231, DMSO treated, 1% O2, 4 hours, biological rep3 |
| GSM2651990 |
MDA-MB-231,MKC-8866 treated, 1% O2, 4 hours, biological rep1 |
| GSM2651991 |
MDA-MB-231,MKC-8866 treated, 1% O2, 4 hours, biological rep2 |
| GSM2651992 |
MDA-MB-231,MKC-8866 treated, 1% O2, 4 hours, biological rep3 |
| GSM2651993 |
MDA-MB-231,MKC-8866 treated, 1% O2, 24 hours, biological rep1 |
| GSM2651994 |
MDA-MB-231,MKC-8866 treated, 1% O2, 24 hours, biological rep2 |
| GSM2651995 |
MDA-MB-231,MKC-8866 treated, 1% O2, 24 hours, biological rep3 |
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| Relations |
| BioProject |
PRJNA389571 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99766_RAW.tar |
160.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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