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Sample GSM1003493 Query DataSets for GSM1003493
Status Public on Sep 13, 2012
Title Broad_ChipSeq_A549_H3K27ac_DEX_100nM
Sample type SRA
 
Source name A549
Organism Homo sapiens
Characteristics biomaterial_type: immortalized cell line
line: A549
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: abcam
antibody vendorid: ab4729
controlid: wgEncodeEH003075
replicate: 1,2
softwareversion: ScriptureVPaperR3
cell sex: M
antibody: H3K27ac
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: Abcam
antibody vendorid: ab4729
treatment: DEX_100nM
treatment description: 1 h with 100 nM Dexamethasone (Myers)
control: std
control description: Standard input signal for most experiments.
controlid: A549/DEX_100nM/Input/std
labversion: Illumina_HiSeq_2000
labversion description: Illumina HiSeq 2000
softwareversion: ScriptureVPaperR3
Biomaterial provider ATCC
Treatment protocol DEX_100nM
Extracted molecule genomic DNA
Extraction protocol Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Data processing http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
 
Submission date Sep 13, 2012
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL11154
Series (1)
GSE29611 Histone Modifications by ChIP-seq from ENCODE/Broad Institute
Relations
SRA SRX186689
BioSample SAMN01174105
Named Annotation GSM1003493_hg19_wgEncodeBroadHistoneA549H3k27acDex100nmSig.bigWig

Supplementary file Size Download File type/resource
GSM1003493_hg19_wgEncodeBroadHistoneA549H3k27acDex100nmPk.broadPeak.gz 849.4 Kb (ftp)(http) BROADPEAK
GSM1003493_hg19_wgEncodeBroadHistoneA549H3k27acDex100nmSig.bigWig 400.2 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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