|
| Status |
Public on Sep 13, 2012 |
| Title |
Broad_ChipSeq_K562_CBX3_(SC-101004) |
| Sample type |
SRA |
| |
|
| Source name |
K562
|
| Organism |
Homo sapiens |
| Characteristics |
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: Mouse monoclonal IgG, raised against recombinant CBX3 of human origin. Antibody Target: CBX3
antibody targetdescription: At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-101004
controlid: wgEncodeEH000052
replicate: 1
softwareversion: ScriptureVPaperR3
cell sex: F
antibody: CBX3_(SC-101004)
antibody antibodydescription: Mouse monoclonal IgG, raised against recombinant CBX3 of human origin. Antibody Target: CBX3
antibody targetdescription: At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-101004
treatment: None
treatment description: No special treatment or protocol applies
control: std
control description: Standard input signal for most experiments.
controlid: K562/None/Input/std
labversion: Illumina_GA2x
labversion description: Illumina Genome Analyzer IIx
softwareversion: ScriptureVPaperR3
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
None
|
| Growth protocol |
K562_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
|
| |
|
| Submission date |
Sep 13, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL10999 |
| Series (2) |
| GSE29611 |
Histone Modifications by ChIP-seq from ENCODE/Broad Institute |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
| Relations |
| SRA |
SRX186764 |
| BioSample |
SAMN01174180 |
| Named Annotation |
GSM1003568_hg19_wgEncodeBroadHistoneK562Cbx3sc101004Sig.bigWig |
