|
Status |
Public on Feb 01, 2013 |
Title |
blood draw 1 from healthy human donor 8 processed by EPCAM CTC chip |
Sample type |
SRA |
|
|
Source name |
blood
|
Organism |
Homo sapiens |
Characteristics |
donor: healthy human donor 8 tissue: blood blood draw: 1 blood processed by: EPCAM CTC chip
|
Extracted molecule |
total RNA |
Extraction protocol |
CTC capture using microfluidic chip. Qiagen RNeasy MinElute kit followed by Helicos single molecule sequencing: Pubmed ID 18388294, 20133332
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Helicos HeliScope |
|
|
Description |
Sample ID: row13.EPCAM mRNA Helicos Digital Gene Expression pipeline. See pubmed ID 19581875
|
Data processing |
Helicos Digital Gene Expression pipeline. Pubmed ID 19581875 Genome_build: hg18 Supplementary_files_format_and_content: The processed_data.txt file is a tab-delimited text file of aligned read counts. Rownames are UCSC knownGene transcript IDs or gene symbols, column names are sample IDs. The processed_data.txt file is linked to the Series record as a supplementary file.
|
|
|
Submission date |
Oct 01, 2012 |
Last update date |
May 15, 2019 |
Contact name |
Ben S. Wittner |
E-mail(s) |
wittner.ben@mgh.harvard.edu
|
Organization name |
Massachusetts General Hospital
|
Department |
Center for Cancer Research
|
Lab |
Lawrence
|
Street address |
149 13th Street
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02129 |
Country |
USA |
|
|
Platform ID |
GPL14761 |
Series (1) |
GSE41245 |
Circulating Breast Tumor Cells Exhibit Dynamic Changes in Epithelial and Mesenchymal Cell Composition |
|
Relations |
SRA |
SRX190450 |
BioSample |
SAMN01737262 |