|
| Status |
Public on Oct 10, 2013 |
| Title |
m175 sorted B |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
m175 sorted
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: co-cultivated for 24 hrs
cell type: H1299-175 co-cultivated with CAFs
|
| Growth protocol |
Cells were grown alone or in co-cultivation for 24h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Tri-Reagent (MRC Inc.) according to manufacturer’s protocol.
|
| Label |
Cy5
|
| Label protocol |
According to manufacturer’s protocol.
|
| |
|
| Channel 2 |
| Source name |
Common reference
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: co-cultivated for 24 hrs
sample type: reference
|
| Growth protocol |
Cells were grown alone or in co-cultivation for 24h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Tri-Reagent (MRC Inc.) according to manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
According to manufacturer’s protocol.
|
| |
|
| |
| Hybridization protocol |
According to manufacturer’s protocol.
|
| Scan protocol |
According to manufacturer’s protocol.
|
| Description |
H1299-175 co-cultivated with CAFs 2 replicates out of 2
|
| Data processing |
The limma package (Smyth and Speed, 2003) was used for microarray processing. Background was corrected using the function backgroundCorrect and normalization within and between arrays was performed using the functions normalizeWithinArrays and normalizeBetweenArrays, respectively. Spots with the same probes were averaged. Analysis of variance (ANOVA) including contrasts was applied to the data set using Partek Genomic Suite 6.5 (Inc. St. Charles, MO).
|
| |
|
| Submission date |
Oct 10, 2012 |
| Last update date |
Oct 10, 2013 |
| Contact name |
Shlomi Madar |
| Organization name |
Weizmann institute of science
|
| Street address |
Hertzl
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE41477 |
Mutant p53 attenuates the anti-tumorigenic activity of fibroblasts secreted interferon (IFN) beta |
|
