|
| Status |
Public on Jun 20, 2013 |
| Title |
miR1225 transfected MEG-01, biological replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
Meg-01 cells, post-nucleofection
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Meg-01 cells
genotype/variation: miR1225 overexpression
|
| Treatment protocol |
Cultured MEG-01 cells were transfected with 200nM of upregulated pre-miRNA 1225-3p or negative control and left for incubation for 24 hours
|
| Growth protocol |
The cells were subcultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin under 5% CO2 at 37 degrees Celsius.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled RNA was prepared according to the standard Agilent protocol from 100ng of total RNA
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 65C on Agilent 8X60K microarray. Arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x60K array slides
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters.
LincRNAs have been excluded from the normalization. Positive and negative controls are also removed.
|
| |
|
| Submission date |
Oct 15, 2012 |
| Last update date |
Jun 20, 2013 |
| Contact name |
Shilpa Jain |
| E-mail(s) |
shilpa.jain3@chp.edu
|
| Organization name |
Uinversity of Pittsburgh
|
| Street address |
200 Lothrop Street
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE41573 |
Gene expression data in Meg-01 cells following transfection with platelet-enriched microRNA-1225-3p |
| GSE41575 |
Role of platelet micrornas in sickle cell disease |
|
